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    Structured Review

    Innoprot Inc spain cat
    Spain Cat, supplied by Innoprot Inc, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spain+cat/Human+Hepatocytes/pmc10772641__mmc35-256-290-288
    Average 92 stars, based on 13 article reviews
    spain cat - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Akt (Ser473) Antibody Cell Signaling technology, Danvers, MA, USA Cat#9271; RRID:AB_329825 Secondary antibody anti-rabbit HRP (horseradish peroxidase) Millipore Sigma, Darmstadt, Germany Cat#12-348; RRID:AB_390191 Anti-tubulin hFAB rhodamine BioRad, Hercules, CA, USA Cat#64225333; RRID:AB_2884950 Akt (pan) (11E7) Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4685; RRID:AB_2225340 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4060; RRID:AB_2315049 b-Actin Antibody (C4) Santa Cruz Biotechnology, Inc; Dallas, TX, USA Cat#sc-47778; RRID:AB_626632 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68070; RRID:AB_10956588 IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-32211; RRID:AB_621843 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68071; RRID:AB_10956166 Biological samples Human body fluids (feces, plasma) This paper N/A Human liver biopsy This paper N/A Mice body fluids (feces, serum) This paper N/A Mice liver This paper N/A Chemicals, peptides, and recombinant proteins Methanol LC-MS Scharlau Cat#ME03262500 Lysing Matrix E MP biomedicals Cat#SKU116914050-CF Acetic acid LC-MS Scharlau Cat#AC03470050 Critical commercial assays QIAamp DNA Stool Mini kit QIAGEN Cat#51504 Bioscientific PCR free library kit Illumina N/A Quant-iT dsDNA Assay Kit, high sensitivity kit Invitrogen Cat#10164582 Agilent Low Input Quick Amp Labeling Kit Agilent Cat#5190-2305 Agilent Gene Expression Hybridization Kit Agilent Cat#5188-5242 Mouse Insulin ELISA Kit Mercodia Cat#10-1247-01 Glucose Liquid Kit QCA Cat#998225 Triglycerides Liquid Kit QCA Cat#992330 Total Cholesterol Liquid Kit QCA Cat#995282 Nextera XT Index Kit v2 Illumina Cat#FC-131-2001 MiSeq Reagent Kit v3 600 cycle Illumina Cat#MS-102-3003 RNeasy Mini Kit QIAGEN Cat#74104 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 AST and ALT colorimetric assays QCA Cat#999500 and 999200 RC/DC Protein Assay Bio-Rad Laboratories Cat#5000121 Pierce BCA Protein assay Thermo Fisher Scientific Cat#23225 Experimental models: Cell lines Primary human hepatocytes Innoprot, Bizkaia, Spain Cat#P10651 (Continued on next page) Cell Reports Medicine 4, 101341, December 19, 2023 e1

    Mouse Assay:

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Akt (Ser473) Antibody Cell Signaling technology, Danvers, MA, USA Cat#9271; RRID:AB_329825 Secondary antibody anti-rabbit HRP (horseradish peroxidase) Millipore Sigma, Darmstadt, Germany Cat#12-348; RRID:AB_390191 Anti-tubulin hFAB rhodamine BioRad, Hercules, CA, USA Cat#64225333; RRID:AB_2884950 Akt (pan) (11E7) Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4685; RRID:AB_2225340 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4060; RRID:AB_2315049 b-Actin Antibody (C4) Santa Cruz Biotechnology, Inc; Dallas, TX, USA Cat#sc-47778; RRID:AB_626632 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68070; RRID:AB_10956588 IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-32211; RRID:AB_621843 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68071; RRID:AB_10956166 Biological samples Human body fluids (feces, plasma) This paper N/A Human liver biopsy This paper N/A Mice body fluids (feces, serum) This paper N/A Mice liver This paper N/A Chemicals, peptides, and recombinant proteins Methanol LC-MS Scharlau Cat#ME03262500 Lysing Matrix E MP biomedicals Cat#SKU116914050-CF Acetic acid LC-MS Scharlau Cat#AC03470050 Critical commercial assays QIAamp DNA Stool Mini kit QIAGEN Cat#51504 Bioscientific PCR free library kit Illumina N/A Quant-iT dsDNA Assay Kit, high sensitivity kit Invitrogen Cat#10164582 Agilent Low Input Quick Amp Labeling Kit Agilent Cat#5190-2305 Agilent Gene Expression Hybridization Kit Agilent Cat#5188-5242 Mouse Insulin ELISA Kit Mercodia Cat#10-1247-01 Glucose Liquid Kit QCA Cat#998225 Triglycerides Liquid Kit QCA Cat#992330 Total Cholesterol Liquid Kit QCA Cat#995282 Nextera XT Index Kit v2 Illumina Cat#FC-131-2001 MiSeq Reagent Kit v3 600 cycle Illumina Cat#MS-102-3003 RNeasy Mini Kit QIAGEN Cat#74104 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 AST and ALT colorimetric assays QCA Cat#999500 and 999200 RC/DC Protein Assay Bio-Rad Laboratories Cat#5000121 Pierce BCA Protein assay Thermo Fisher Scientific Cat#23225 Experimental models: Cell lines Primary human hepatocytes Innoprot, Bizkaia, Spain Cat#P10651 (Continued on next page) Cell Reports Medicine 4, 101341, December 19, 2023 e1

    Recombinant:

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Akt (Ser473) Antibody Cell Signaling technology, Danvers, MA, USA Cat#9271; RRID:AB_329825 Secondary antibody anti-rabbit HRP (horseradish peroxidase) Millipore Sigma, Darmstadt, Germany Cat#12-348; RRID:AB_390191 Anti-tubulin hFAB rhodamine BioRad, Hercules, CA, USA Cat#64225333; RRID:AB_2884950 Akt (pan) (11E7) Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4685; RRID:AB_2225340 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4060; RRID:AB_2315049 b-Actin Antibody (C4) Santa Cruz Biotechnology, Inc; Dallas, TX, USA Cat#sc-47778; RRID:AB_626632 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68070; RRID:AB_10956588 IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-32211; RRID:AB_621843 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68071; RRID:AB_10956166 Biological samples Human body fluids (feces, plasma) This paper N/A Human liver biopsy This paper N/A Mice body fluids (feces, serum) This paper N/A Mice liver This paper N/A Chemicals, peptides, and recombinant proteins Methanol LC-MS Scharlau Cat#ME03262500 Lysing Matrix E MP biomedicals Cat#SKU116914050-CF Acetic acid LC-MS Scharlau Cat#AC03470050 Critical commercial assays QIAamp DNA Stool Mini kit QIAGEN Cat#51504 Bioscientific PCR free library kit Illumina N/A Quant-iT dsDNA Assay Kit, high sensitivity kit Invitrogen Cat#10164582 Agilent Low Input Quick Amp Labeling Kit Agilent Cat#5190-2305 Agilent Gene Expression Hybridization Kit Agilent Cat#5188-5242 Mouse Insulin ELISA Kit Mercodia Cat#10-1247-01 Glucose Liquid Kit QCA Cat#998225 Triglycerides Liquid Kit QCA Cat#992330 Total Cholesterol Liquid Kit QCA Cat#995282 Nextera XT Index Kit v2 Illumina Cat#FC-131-2001 MiSeq Reagent Kit v3 600 cycle Illumina Cat#MS-102-3003 RNeasy Mini Kit QIAGEN Cat#74104 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 AST and ALT colorimetric assays QCA Cat#999500 and 999200 RC/DC Protein Assay Bio-Rad Laboratories Cat#5000121 Pierce BCA Protein assay Thermo Fisher Scientific Cat#23225 Experimental models: Cell lines Primary human hepatocytes Innoprot, Bizkaia, Spain Cat#P10651 (Continued on next page) Cell Reports Medicine 4, 101341, December 19, 2023 e1

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Akt (Ser473) Antibody Cell Signaling technology, Danvers, MA, USA Cat#9271; RRID:AB_329825 Secondary antibody anti-rabbit HRP (horseradish peroxidase) Millipore Sigma, Darmstadt, Germany Cat#12-348; RRID:AB_390191 Anti-tubulin hFAB rhodamine BioRad, Hercules, CA, USA Cat#64225333; RRID:AB_2884950 Akt (pan) (11E7) Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4685; RRID:AB_2225340 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4060; RRID:AB_2315049 b-Actin Antibody (C4) Santa Cruz Biotechnology, Inc; Dallas, TX, USA Cat#sc-47778; RRID:AB_626632 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68070; RRID:AB_10956588 IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-32211; RRID:AB_621843 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68071; RRID:AB_10956166 Biological samples Human body fluids (feces, plasma) This paper N/A Human liver biopsy This paper N/A Mice body fluids (feces, serum) This paper N/A Mice liver This paper N/A Chemicals, peptides, and recombinant proteins Methanol LC-MS Scharlau Cat#ME03262500 Lysing Matrix E MP biomedicals Cat#SKU116914050-CF Acetic acid LC-MS Scharlau Cat#AC03470050 Critical commercial assays QIAamp DNA Stool Mini kit QIAGEN Cat#51504 Bioscientific PCR free library kit Illumina N/A Quant-iT dsDNA Assay Kit, high sensitivity kit Invitrogen Cat#10164582 Agilent Low Input Quick Amp Labeling Kit Agilent Cat#5190-2305 Agilent Gene Expression Hybridization Kit Agilent Cat#5188-5242 Mouse Insulin ELISA Kit Mercodia Cat#10-1247-01 Glucose Liquid Kit QCA Cat#998225 Triglycerides Liquid Kit QCA Cat#992330 Total Cholesterol Liquid Kit QCA Cat#995282 Nextera XT Index Kit v2 Illumina Cat#FC-131-2001 MiSeq Reagent Kit v3 600 cycle Illumina Cat#MS-102-3003 RNeasy Mini Kit QIAGEN Cat#74104 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 AST and ALT colorimetric assays QCA Cat#999500 and 999200 RC/DC Protein Assay Bio-Rad Laboratories Cat#5000121 Pierce BCA Protein assay Thermo Fisher Scientific Cat#23225 Experimental models: Cell lines Primary human hepatocytes Innoprot, Bizkaia, Spain Cat#P10651 (Continued on next page) Cell Reports Medicine 4, 101341, December 19, 2023 e1

    Polymerase Chain Reaction:

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Akt (Ser473) Antibody Cell Signaling technology, Danvers, MA, USA Cat#9271; RRID:AB_329825 Secondary antibody anti-rabbit HRP (horseradish peroxidase) Millipore Sigma, Darmstadt, Germany Cat#12-348; RRID:AB_390191 Anti-tubulin hFAB rhodamine BioRad, Hercules, CA, USA Cat#64225333; RRID:AB_2884950 Akt (pan) (11E7) Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4685; RRID:AB_2225340 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4060; RRID:AB_2315049 b-Actin Antibody (C4) Santa Cruz Biotechnology, Inc; Dallas, TX, USA Cat#sc-47778; RRID:AB_626632 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68070; RRID:AB_10956588 IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-32211; RRID:AB_621843 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68071; RRID:AB_10956166 Biological samples Human body fluids (feces, plasma) This paper N/A Human liver biopsy This paper N/A Mice body fluids (feces, serum) This paper N/A Mice liver This paper N/A Chemicals, peptides, and recombinant proteins Methanol LC-MS Scharlau Cat#ME03262500 Lysing Matrix E MP biomedicals Cat#SKU116914050-CF Acetic acid LC-MS Scharlau Cat#AC03470050 Critical commercial assays QIAamp DNA Stool Mini kit QIAGEN Cat#51504 Bioscientific PCR free library kit Illumina N/A Quant-iT dsDNA Assay Kit, high sensitivity kit Invitrogen Cat#10164582 Agilent Low Input Quick Amp Labeling Kit Agilent Cat#5190-2305 Agilent Gene Expression Hybridization Kit Agilent Cat#5188-5242 Mouse Insulin ELISA Kit Mercodia Cat#10-1247-01 Glucose Liquid Kit QCA Cat#998225 Triglycerides Liquid Kit QCA Cat#992330 Total Cholesterol Liquid Kit QCA Cat#995282 Nextera XT Index Kit v2 Illumina Cat#FC-131-2001 MiSeq Reagent Kit v3 600 cycle Illumina Cat#MS-102-3003 RNeasy Mini Kit QIAGEN Cat#74104 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 AST and ALT colorimetric assays QCA Cat#999500 and 999200 RC/DC Protein Assay Bio-Rad Laboratories Cat#5000121 Pierce BCA Protein assay Thermo Fisher Scientific Cat#23225 Experimental models: Cell lines Primary human hepatocytes Innoprot, Bizkaia, Spain Cat#P10651 (Continued on next page) Cell Reports Medicine 4, 101341, December 19, 2023 e1

    dsDNA Assay:

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Akt (Ser473) Antibody Cell Signaling technology, Danvers, MA, USA Cat#9271; RRID:AB_329825 Secondary antibody anti-rabbit HRP (horseradish peroxidase) Millipore Sigma, Darmstadt, Germany Cat#12-348; RRID:AB_390191 Anti-tubulin hFAB rhodamine BioRad, Hercules, CA, USA Cat#64225333; RRID:AB_2884950 Akt (pan) (11E7) Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4685; RRID:AB_2225340 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4060; RRID:AB_2315049 b-Actin Antibody (C4) Santa Cruz Biotechnology, Inc; Dallas, TX, USA Cat#sc-47778; RRID:AB_626632 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68070; RRID:AB_10956588 IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-32211; RRID:AB_621843 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68071; RRID:AB_10956166 Biological samples Human body fluids (feces, plasma) This paper N/A Human liver biopsy This paper N/A Mice body fluids (feces, serum) This paper N/A Mice liver This paper N/A Chemicals, peptides, and recombinant proteins Methanol LC-MS Scharlau Cat#ME03262500 Lysing Matrix E MP biomedicals Cat#SKU116914050-CF Acetic acid LC-MS Scharlau Cat#AC03470050 Critical commercial assays QIAamp DNA Stool Mini kit QIAGEN Cat#51504 Bioscientific PCR free library kit Illumina N/A Quant-iT dsDNA Assay Kit, high sensitivity kit Invitrogen Cat#10164582 Agilent Low Input Quick Amp Labeling Kit Agilent Cat#5190-2305 Agilent Gene Expression Hybridization Kit Agilent Cat#5188-5242 Mouse Insulin ELISA Kit Mercodia Cat#10-1247-01 Glucose Liquid Kit QCA Cat#998225 Triglycerides Liquid Kit QCA Cat#992330 Total Cholesterol Liquid Kit QCA Cat#995282 Nextera XT Index Kit v2 Illumina Cat#FC-131-2001 MiSeq Reagent Kit v3 600 cycle Illumina Cat#MS-102-3003 RNeasy Mini Kit QIAGEN Cat#74104 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 AST and ALT colorimetric assays QCA Cat#999500 and 999200 RC/DC Protein Assay Bio-Rad Laboratories Cat#5000121 Pierce BCA Protein assay Thermo Fisher Scientific Cat#23225 Experimental models: Cell lines Primary human hepatocytes Innoprot, Bizkaia, Spain Cat#P10651 (Continued on next page) Cell Reports Medicine 4, 101341, December 19, 2023 e1

    Labeling:

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Akt (Ser473) Antibody Cell Signaling technology, Danvers, MA, USA Cat#9271; RRID:AB_329825 Secondary antibody anti-rabbit HRP (horseradish peroxidase) Millipore Sigma, Darmstadt, Germany Cat#12-348; RRID:AB_390191 Anti-tubulin hFAB rhodamine BioRad, Hercules, CA, USA Cat#64225333; RRID:AB_2884950 Akt (pan) (11E7) Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4685; RRID:AB_2225340 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4060; RRID:AB_2315049 b-Actin Antibody (C4) Santa Cruz Biotechnology, Inc; Dallas, TX, USA Cat#sc-47778; RRID:AB_626632 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68070; RRID:AB_10956588 IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-32211; RRID:AB_621843 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68071; RRID:AB_10956166 Biological samples Human body fluids (feces, plasma) This paper N/A Human liver biopsy This paper N/A Mice body fluids (feces, serum) This paper N/A Mice liver This paper N/A Chemicals, peptides, and recombinant proteins Methanol LC-MS Scharlau Cat#ME03262500 Lysing Matrix E MP biomedicals Cat#SKU116914050-CF Acetic acid LC-MS Scharlau Cat#AC03470050 Critical commercial assays QIAamp DNA Stool Mini kit QIAGEN Cat#51504 Bioscientific PCR free library kit Illumina N/A Quant-iT dsDNA Assay Kit, high sensitivity kit Invitrogen Cat#10164582 Agilent Low Input Quick Amp Labeling Kit Agilent Cat#5190-2305 Agilent Gene Expression Hybridization Kit Agilent Cat#5188-5242 Mouse Insulin ELISA Kit Mercodia Cat#10-1247-01 Glucose Liquid Kit QCA Cat#998225 Triglycerides Liquid Kit QCA Cat#992330 Total Cholesterol Liquid Kit QCA Cat#995282 Nextera XT Index Kit v2 Illumina Cat#FC-131-2001 MiSeq Reagent Kit v3 600 cycle Illumina Cat#MS-102-3003 RNeasy Mini Kit QIAGEN Cat#74104 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 AST and ALT colorimetric assays QCA Cat#999500 and 999200 RC/DC Protein Assay Bio-Rad Laboratories Cat#5000121 Pierce BCA Protein assay Thermo Fisher Scientific Cat#23225 Experimental models: Cell lines Primary human hepatocytes Innoprot, Bizkaia, Spain Cat#P10651 (Continued on next page) Cell Reports Medicine 4, 101341, December 19, 2023 e1

    Gene Expression:

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Akt (Ser473) Antibody Cell Signaling technology, Danvers, MA, USA Cat#9271; RRID:AB_329825 Secondary antibody anti-rabbit HRP (horseradish peroxidase) Millipore Sigma, Darmstadt, Germany Cat#12-348; RRID:AB_390191 Anti-tubulin hFAB rhodamine BioRad, Hercules, CA, USA Cat#64225333; RRID:AB_2884950 Akt (pan) (11E7) Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4685; RRID:AB_2225340 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4060; RRID:AB_2315049 b-Actin Antibody (C4) Santa Cruz Biotechnology, Inc; Dallas, TX, USA Cat#sc-47778; RRID:AB_626632 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68070; RRID:AB_10956588 IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-32211; RRID:AB_621843 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68071; RRID:AB_10956166 Biological samples Human body fluids (feces, plasma) This paper N/A Human liver biopsy This paper N/A Mice body fluids (feces, serum) This paper N/A Mice liver This paper N/A Chemicals, peptides, and recombinant proteins Methanol LC-MS Scharlau Cat#ME03262500 Lysing Matrix E MP biomedicals Cat#SKU116914050-CF Acetic acid LC-MS Scharlau Cat#AC03470050 Critical commercial assays QIAamp DNA Stool Mini kit QIAGEN Cat#51504 Bioscientific PCR free library kit Illumina N/A Quant-iT dsDNA Assay Kit, high sensitivity kit Invitrogen Cat#10164582 Agilent Low Input Quick Amp Labeling Kit Agilent Cat#5190-2305 Agilent Gene Expression Hybridization Kit Agilent Cat#5188-5242 Mouse Insulin ELISA Kit Mercodia Cat#10-1247-01 Glucose Liquid Kit QCA Cat#998225 Triglycerides Liquid Kit QCA Cat#992330 Total Cholesterol Liquid Kit QCA Cat#995282 Nextera XT Index Kit v2 Illumina Cat#FC-131-2001 MiSeq Reagent Kit v3 600 cycle Illumina Cat#MS-102-3003 RNeasy Mini Kit QIAGEN Cat#74104 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 AST and ALT colorimetric assays QCA Cat#999500 and 999200 RC/DC Protein Assay Bio-Rad Laboratories Cat#5000121 Pierce BCA Protein assay Thermo Fisher Scientific Cat#23225 Experimental models: Cell lines Primary human hepatocytes Innoprot, Bizkaia, Spain Cat#P10651 (Continued on next page) Cell Reports Medicine 4, 101341, December 19, 2023 e1

    Hybridization:

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Akt (Ser473) Antibody Cell Signaling technology, Danvers, MA, USA Cat#9271; RRID:AB_329825 Secondary antibody anti-rabbit HRP (horseradish peroxidase) Millipore Sigma, Darmstadt, Germany Cat#12-348; RRID:AB_390191 Anti-tubulin hFAB rhodamine BioRad, Hercules, CA, USA Cat#64225333; RRID:AB_2884950 Akt (pan) (11E7) Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4685; RRID:AB_2225340 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4060; RRID:AB_2315049 b-Actin Antibody (C4) Santa Cruz Biotechnology, Inc; Dallas, TX, USA Cat#sc-47778; RRID:AB_626632 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68070; RRID:AB_10956588 IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-32211; RRID:AB_621843 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68071; RRID:AB_10956166 Biological samples Human body fluids (feces, plasma) This paper N/A Human liver biopsy This paper N/A Mice body fluids (feces, serum) This paper N/A Mice liver This paper N/A Chemicals, peptides, and recombinant proteins Methanol LC-MS Scharlau Cat#ME03262500 Lysing Matrix E MP biomedicals Cat#SKU116914050-CF Acetic acid LC-MS Scharlau Cat#AC03470050 Critical commercial assays QIAamp DNA Stool Mini kit QIAGEN Cat#51504 Bioscientific PCR free library kit Illumina N/A Quant-iT dsDNA Assay Kit, high sensitivity kit Invitrogen Cat#10164582 Agilent Low Input Quick Amp Labeling Kit Agilent Cat#5190-2305 Agilent Gene Expression Hybridization Kit Agilent Cat#5188-5242 Mouse Insulin ELISA Kit Mercodia Cat#10-1247-01 Glucose Liquid Kit QCA Cat#998225 Triglycerides Liquid Kit QCA Cat#992330 Total Cholesterol Liquid Kit QCA Cat#995282 Nextera XT Index Kit v2 Illumina Cat#FC-131-2001 MiSeq Reagent Kit v3 600 cycle Illumina Cat#MS-102-3003 RNeasy Mini Kit QIAGEN Cat#74104 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 AST and ALT colorimetric assays QCA Cat#999500 and 999200 RC/DC Protein Assay Bio-Rad Laboratories Cat#5000121 Pierce BCA Protein assay Thermo Fisher Scientific Cat#23225 Experimental models: Cell lines Primary human hepatocytes Innoprot, Bizkaia, Spain Cat#P10651 (Continued on next page) Cell Reports Medicine 4, 101341, December 19, 2023 e1

    Enzyme-linked Immunosorbent Assay:

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Akt (Ser473) Antibody Cell Signaling technology, Danvers, MA, USA Cat#9271; RRID:AB_329825 Secondary antibody anti-rabbit HRP (horseradish peroxidase) Millipore Sigma, Darmstadt, Germany Cat#12-348; RRID:AB_390191 Anti-tubulin hFAB rhodamine BioRad, Hercules, CA, USA Cat#64225333; RRID:AB_2884950 Akt (pan) (11E7) Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4685; RRID:AB_2225340 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4060; RRID:AB_2315049 b-Actin Antibody (C4) Santa Cruz Biotechnology, Inc; Dallas, TX, USA Cat#sc-47778; RRID:AB_626632 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68070; RRID:AB_10956588 IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-32211; RRID:AB_621843 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68071; RRID:AB_10956166 Biological samples Human body fluids (feces, plasma) This paper N/A Human liver biopsy This paper N/A Mice body fluids (feces, serum) This paper N/A Mice liver This paper N/A Chemicals, peptides, and recombinant proteins Methanol LC-MS Scharlau Cat#ME03262500 Lysing Matrix E MP biomedicals Cat#SKU116914050-CF Acetic acid LC-MS Scharlau Cat#AC03470050 Critical commercial assays QIAamp DNA Stool Mini kit QIAGEN Cat#51504 Bioscientific PCR free library kit Illumina N/A Quant-iT dsDNA Assay Kit, high sensitivity kit Invitrogen Cat#10164582 Agilent Low Input Quick Amp Labeling Kit Agilent Cat#5190-2305 Agilent Gene Expression Hybridization Kit Agilent Cat#5188-5242 Mouse Insulin ELISA Kit Mercodia Cat#10-1247-01 Glucose Liquid Kit QCA Cat#998225 Triglycerides Liquid Kit QCA Cat#992330 Total Cholesterol Liquid Kit QCA Cat#995282 Nextera XT Index Kit v2 Illumina Cat#FC-131-2001 MiSeq Reagent Kit v3 600 cycle Illumina Cat#MS-102-3003 RNeasy Mini Kit QIAGEN Cat#74104 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 AST and ALT colorimetric assays QCA Cat#999500 and 999200 RC/DC Protein Assay Bio-Rad Laboratories Cat#5000121 Pierce BCA Protein assay Thermo Fisher Scientific Cat#23225 Experimental models: Cell lines Primary human hepatocytes Innoprot, Bizkaia, Spain Cat#P10651 (Continued on next page) Cell Reports Medicine 4, 101341, December 19, 2023 e1

    AST Assay:

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Akt (Ser473) Antibody Cell Signaling technology, Danvers, MA, USA Cat#9271; RRID:AB_329825 Secondary antibody anti-rabbit HRP (horseradish peroxidase) Millipore Sigma, Darmstadt, Germany Cat#12-348; RRID:AB_390191 Anti-tubulin hFAB rhodamine BioRad, Hercules, CA, USA Cat#64225333; RRID:AB_2884950 Akt (pan) (11E7) Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4685; RRID:AB_2225340 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4060; RRID:AB_2315049 b-Actin Antibody (C4) Santa Cruz Biotechnology, Inc; Dallas, TX, USA Cat#sc-47778; RRID:AB_626632 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68070; RRID:AB_10956588 IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-32211; RRID:AB_621843 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68071; RRID:AB_10956166 Biological samples Human body fluids (feces, plasma) This paper N/A Human liver biopsy This paper N/A Mice body fluids (feces, serum) This paper N/A Mice liver This paper N/A Chemicals, peptides, and recombinant proteins Methanol LC-MS Scharlau Cat#ME03262500 Lysing Matrix E MP biomedicals Cat#SKU116914050-CF Acetic acid LC-MS Scharlau Cat#AC03470050 Critical commercial assays QIAamp DNA Stool Mini kit QIAGEN Cat#51504 Bioscientific PCR free library kit Illumina N/A Quant-iT dsDNA Assay Kit, high sensitivity kit Invitrogen Cat#10164582 Agilent Low Input Quick Amp Labeling Kit Agilent Cat#5190-2305 Agilent Gene Expression Hybridization Kit Agilent Cat#5188-5242 Mouse Insulin ELISA Kit Mercodia Cat#10-1247-01 Glucose Liquid Kit QCA Cat#998225 Triglycerides Liquid Kit QCA Cat#992330 Total Cholesterol Liquid Kit QCA Cat#995282 Nextera XT Index Kit v2 Illumina Cat#FC-131-2001 MiSeq Reagent Kit v3 600 cycle Illumina Cat#MS-102-3003 RNeasy Mini Kit QIAGEN Cat#74104 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 AST and ALT colorimetric assays QCA Cat#999500 and 999200 RC/DC Protein Assay Bio-Rad Laboratories Cat#5000121 Pierce BCA Protein assay Thermo Fisher Scientific Cat#23225 Experimental models: Cell lines Primary human hepatocytes Innoprot, Bizkaia, Spain Cat#P10651 (Continued on next page) Cell Reports Medicine 4, 101341, December 19, 2023 e1

    Bicinchoninic Acid Protein Assay:

    Article Title: Potential therapeutic implications of histidine catabolism by the gut microbiota in NAFLD patients with morbid obesity
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Akt (Ser473) Antibody Cell Signaling technology, Danvers, MA, USA Cat#9271; RRID:AB_329825 Secondary antibody anti-rabbit HRP (horseradish peroxidase) Millipore Sigma, Darmstadt, Germany Cat#12-348; RRID:AB_390191 Anti-tubulin hFAB rhodamine BioRad, Hercules, CA, USA Cat#64225333; RRID:AB_2884950 Akt (pan) (11E7) Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4685; RRID:AB_2225340 Phospho-Akt (Ser473) (D9E) XP Rabbit mAb Cell Signaling technology, Danvers, MA, USA Cat#4060; RRID:AB_2315049 b-Actin Antibody (C4) Santa Cruz Biotechnology, Inc; Dallas, TX, USA Cat#sc-47778; RRID:AB_626632 IRDye 680RD Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68070; RRID:AB_10956588 IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-32211; RRID:AB_621843 IRDye 680RD Goat anti-Mouse IgG Secondary Antibody LI-COR Biosciences, Lincoln, NE, USA Cat#926-68071; RRID:AB_10956166 Biological samples Human body fluids (feces, plasma) This paper N/A Human liver biopsy This paper N/A Mice body fluids (feces, serum) This paper N/A Mice liver This paper N/A Chemicals, peptides, and recombinant proteins Methanol LC-MS Scharlau Cat#ME03262500 Lysing Matrix E MP biomedicals Cat#SKU116914050-CF Acetic acid LC-MS Scharlau Cat#AC03470050 Critical commercial assays QIAamp DNA Stool Mini kit QIAGEN Cat#51504 Bioscientific PCR free library kit Illumina N/A Quant-iT dsDNA Assay Kit, high sensitivity kit Invitrogen Cat#10164582 Agilent Low Input Quick Amp Labeling Kit Agilent Cat#5190-2305 Agilent Gene Expression Hybridization Kit Agilent Cat#5188-5242 Mouse Insulin ELISA Kit Mercodia Cat#10-1247-01 Glucose Liquid Kit QCA Cat#998225 Triglycerides Liquid Kit QCA Cat#992330 Total Cholesterol Liquid Kit QCA Cat#995282 Nextera XT Index Kit v2 Illumina Cat#FC-131-2001 MiSeq Reagent Kit v3 600 cycle Illumina Cat#MS-102-3003 RNeasy Mini Kit QIAGEN Cat#74104 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4387406 AST and ALT colorimetric assays QCA Cat#999500 and 999200 RC/DC Protein Assay Bio-Rad Laboratories Cat#5000121 Pierce BCA Protein assay Thermo Fisher Scientific Cat#23225 Experimental models: Cell lines Primary human hepatocytes Innoprot, Bizkaia, Spain Cat#P10651 (Continued on next page) Cell Reports Medicine 4, 101341, December 19, 2023 e1



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